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- Title
Skin-Layer Formation on Porous Membrane by Immobilized Dextransucrase.
- Authors
Kawakita, Hidetaka; Saito, Kyoichi; Sugita, Kazuyuki; Tamada, Masao; Sugo, Takanobu; Kawamoto, Hiroshi
- Abstract
A porous hollow fiber membrane with an average pore diameter of 360 nm and a porosity of 71% was used as a starting polymer. An epoxy-group-containing monomer, glycidyl methacrylate, was grafted onto the porous hollow fiber membrane by radiation-induced graft polymerization. The produced epoxy group was quantitatively converted into a 2-hydroxyethylamino group as an anion-exchange group by reaction with ethanolamine. Dextransucrase solution was forced to permeate radially outward through the pore from the inside surface of the porous hollow-fiber membrane to be immobilized by the grafted polymer chains based on an anion-exchange interaction. Subsequently, sucrose solution as substrate was fed to the inside surface of the dextransucrase- immobilized porous hollow fiber membrane. Dextran produced by the enzymatic reaction formed a skin layer on the inside surface of the membrane with a thickness of approximately 4 µm and an estimated pore diameter of 50 nm.
- Subjects
POROUS materials; POLYMERS; METHYL methacrylate; EPOXY resins; POLYMERIZATION; ANIONS
- Publication
AIChE Journal, 2004, Vol 50, Issue 3, p696
- ISSN
0001-1541
- Publication type
Article
- DOI
10.1002/aic.10063