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- Title
Bacterial detection and extended platelet storage.
- Authors
Arun, A.; Jayaprasad; Sangeetha, A. S.; Zahara, R.
- Abstract
Bacterial contamination of transfusion products, especially platelets, is a long standing problem. Platelet concentrates are stored under aerobic conditions at 22 degrees centigrade; circumstances that are ideal for bacterial growth. The American Association of Blood Banks (AABB) proposed new standards to help mitigate transfusion of units that were contaminated with bacteria. Adopted with a final implementation date of March 1, 2004, the AABB Standard reads .The blood bank or transfusion service shall have methods to limit and detect bacterial contamination in all platelet components.. As required by the drug control department for quality control 1 % of the platelets have to be tested for sterility. Storage and use is permitted for 5 days from the time of collection. In the Indian context it has not yet been made mandatory to check all the products for bacterial contamination as indicated in the AABB standards The cost and availability of facilities is probably a major constraint in doing so. Bacterial testing, however, provides an opportunity for an extension of shelf life of platelet concentrates from 5 to 7 days. This will help hospitals manage their inventories and reduce outdates provided the platelets are collected in approved containers and are tested for bacterial sterility as per US FDA norms. Possible mechanisms of the bacterial contamination of blood components include: donor bacteremia, contamination during the whole blood collection procedure, contamination of the collection pack and contamination during the blood processing procedure. Our study tries to assess the feasibility of routine bacterial testing of all platelets and consequently all blood products and thereby tries to propose extension of shelf life of whole blood derived platelets to 7 days. In the present study 500 consecutive units collected, were cultured after 24 hours of incubation at 22 degrees centigrade and again at seven days of shelf life, for aerobic and anaerobic culture. The study also attempts to evaluate the difference between the use of spirit alone and spirit and iodine scrub, for skin preparation prior to donor blood collection. The observations of this study shall be presented and discussed.
- Subjects
INDIA; MICROBIAL contamination; BLOOD platelet transfusion; BLOOD collection; RISK management in blood banks; QUALITY control; BLOOD transfusion
- Publication
Asian Journal of Transfusion Science, 2007, Vol 1, Issue 2, p94
- ISSN
0973-6247
- Publication type
Article