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- Title
RANDOM-CENTROID OPTIMIZATION OF ULTRASOUND-ASSISTED EXTRACTION OF POLYSACCHARIDES FROM CORDYCEPS SINENSIS MYCELIUM.
- Authors
Jing-Kun Yan; Yao-Yao Wang
- Abstract
Ultrasound-assisted extraction was used to extract crude polysaccharides (PS) from Cordyceps sinensis (Cs-4) mycelium, and the effects of processing parameters on polysaccharide yields were optimized by random-centroid optimization (RCO). The optimal extraction conditions were as follows: liquid to solid ratio of 30 mL/g, ultrasonic power of 550 W, ultrasonic time of 20 min, and extraction time of 3. Under these conditions, the maximum extraction yield of PS was 16.60%. The partially purified PS-1 contained high-molecularweight (730 kDa, 80.86%) and low-molecular-weight (5.8 kDa, 19.14%) polysaccharides composed of D-glucose, D-mannose, and D-galactose in a ratio of 1.8:1.2:1.0 with α-type glycosidic bonds. The results indicated that ultrasound-assisted extraction was a very powerful and efficient tool for the extraction of polysaccharides from fungi or mushrooms, and RCO was an useful approach for experimental variable optimization.
- Subjects
POLYSACCHARIDES; CENTROID; CORDYCEPS; MYCELIUM; MOLECULAR weights; MATHEMATICAL optimization
- Publication
Current Topics in Nutraceutical Research, 2015, Vol 13, Issue 4, p167
- ISSN
1540-7535
- Publication type
Article