We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Use of supplemental anti- HBc testing of donors showing non-discriminating reactive results in multiplex nucleic acid testing.
- Authors
Kang, J. W.; Seo, J. H.; Youn, K. W.; Seo, Y. I.; Huh, K.; Choi, G. R.; Min, H. K.; Oh, D. J.; Jo, H. J.; Kim, J. N.
- Abstract
Background and Objectives The Korean Red Cross began nucleic acid amplification testing ( NAT) for HIV and HCV in February 2005, and added HBV NAT beginning in June 2012. The current NAT system utilizes a multiplex assay for simultaneous detection of HBV DNA, HCV RNA and HIV-1 RNA. For samples that are reactive in the multiplex assay, we do specific tests for each virus. However, there have been cases of non-discriminated reactive ( NDR) results which appear to be the result of non-specific reactions or cross-contamination, although some cases are considered to arise from the presence of low levels of HBV DNA due to occult hepatitis B infection. Materials and Methods We examined the incidence of NDR results in previous donations of some NAT-reactive donors. Additionally, for those donors with NDR results, we performed an HBV core antibody (anti- HBc) assay. Results From November 2015 to March 2016, there were 408 NAT-reactive donors. Of these, nineteen HBV NAT-reactive donors showed a history of NDR results in the past donations. Seven donors showed NDR results more than once. Of 771 NDR donors, 362 (47·0%) were anti- HBc reactive. Conclusion The NDR donors had a substantially higher rate of anti- HBc reactivity than other blood donors indicating that some with anti-HBc reactivity represent donors with occult HBV. Therefore, the incorporation of an anti- HBc testing for NDR donors could improve blood safety testing for the Korean Red Cross.
- Subjects
BLOOD donors; NUCLEIC acid amplification techniques; BLOOD transfusion; HIV infections; HEPATITIS C virus; SAFETY
- Publication
Vox Sanguinis, 2017, Vol 112, Issue 7, p622
- ISSN
0042-9007
- Publication type
Article
- DOI
10.1111/vox.12553