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- Title
Riboflavin and amotosalen photochemical treatments of platelet concentrates reduce thrombus formation kinetics in vitro.
- Authors
Van Aelst, B.; Feys, H. B.; Devloo, R.; Vanhoorelbeke, K.; Vandekerckhove, P.; Compernolle, V.
- Abstract
Background Photochemical treatment ( PCT) of platelet concentrates using photosensitizers and ultraviolet light illumination reduces the proliferation potential of pathogens by damaging biomolecules. Materials and Methods The impact of riboflavin ( RF- PRT)- and amotosalen ( AS- PCT)-based pathogen inactivation on platelets was studied using microfluidic flow chambers on immobilized collagen using standard platelet concentrates prepared from buffy coats in additive solution. Flow cytometry, metabolic parameters and light transmission aggregometry with thrombin-related peptide, collagen and ristocetin were determined concurrently. Results Both PCTs significantly decreased the platelet surface coverage kinetics in flow chambers over the course of the 7-day study. Platelet aggregation was affected following RF- PRT in response to all agonists, while AS- PCT mainly impacted low-dose ristocetin agglutination. RF- PRT induces premature platelet activation because integrin α IIbβ3 was spontaneously activated, and α-degranulation, phosphatidylserine/-ethanolamine exposure and anaerobic metabolism significantly increased following treatment, which was not the case for AS- PCT. On the other hand, AS- PCT significantly diminished thrombus growth onto von Willebrand factor under shear flow. This defect was caused by fewer integrin α IIbβ3 interactions, not by defective GPIbα- VWF binding as shown by adhesion experiments in the presence of tirofiban. Moreover, integrin α IIbβ3 activation was also affected following the activation of platelets via GPVI-Fcγ RIIa or PAR1. Finally, amotosalen illumination as such is sufficient to induce platelet damage, with no additional measurable effect of the chemical adsorption step. Gamma irradiation caused no significant difference compared to controls on any time-point or for any parameter. Conclusion Both PCTs significantly reduce thrombus formation rate but by different biochemical mechanisms.
- Subjects
VITAMIN B2; PHOTOCHEMISTRY; BLOOD platelets; THROMBOSIS; IN vitro studies; MICROFLUIDICS
- Publication
Vox Sanguinis, 2015, Vol 108, Issue 4, p328
- ISSN
0042-9007
- Publication type
Article
- DOI
10.1111/vox.12231