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- Title
Extract from Eugenia punicifolia is an Antioxidant and Inhibits Enzymes Related to Metabolic Syndrome.
- Authors
Galeno, Denise; Carvalho, Rosany; Araújo Boleti, Ana; Lima, Arleilson; Almeida, Patricia; Pacheco, Carolina; Souza, Tatiane; Lima, Emerson
- Abstract
The present study aimed to investigate in vitro biological activities of extract of Eugenia punicifolia leaves (EEP), emphasizing the inhibitory activity of enzymes related to metabolic syndrome and its antioxidant effects. The antioxidant activity was analyzed by free radicals scavengers in vitro assays: DPPH·, ABTS, O, and NO· and a cell-based assay. EEP were tested in inhibitory colorimetric assays using α-amylase, α-glucosidase, xanthine oxidase, and pancreatic lipase enzymes. The EEP exhibited activity in ABTS, DPPH·, and O scavenger (IC = 10.5 ± 1.2, 28.84 ± 0.54, and 38.12 ± 2.6 μg/mL), respectively. EEP did not show cytotoxic effects, and it showed antioxidant activity in cells in a concentration-dependent manner. EEP exhibited inhibition of α-amylase, α-glucosidase, and xanthine oxidase activities in vitro assays (IC = 122.8 ± 6.3; 2.9 ± 0.1; 23.5 ± 2.6), respectively; however, EEP did not inhibit the lipase activity. The findings supported that extract of E. punicifolia leaves is a natural antioxidant and inhibitor of enzymes, such as α-amylase, α-glucosidase, and xanthine oxidase, which can result in a reduction in the carbohydrate absorption rate and decrease of risks factors of cardiovascular disease, thereby providing a novel dietary opportunity for the prevention of metabolic syndrome.
- Subjects
ANTIOXIDANTS; BIOLOGICAL assay; EUGENIA; PLANT enzyme analysis; PLANT extracts; METABOLIC syndrome; COMPOSITION of leaves
- Publication
Applied Biochemistry & Biotechnology, 2014, Vol 172, Issue 1, p311
- ISSN
0273-2289
- Publication type
Article
- DOI
10.1007/s12010-013-0520-8