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- Title
Development of a Modular Ratiometric Fluorescent Probe for the Detection of Extracellular Superoxide.
- Authors
Andina, Diana; Brambilla, Davide; Munzinger, Noah; Frei, Jasmine; Zivko, Cristina; Leroux, Jean‐Christophe; Luciani, Paola
- Abstract
Extracellular detection of endogeneous analytes (e.g., superoxide) can provide important insights into mechanisms of homeostasis and diseases, such as tumorigenesis. A ratiometric probe with a fluorescent reference and an analyte-specific switch-on dye was developed. Detection of ROS in the extracellular milieu was ensured by connecting the two fluorophores with a modular peptide-nucleic-acid-based linker. The ROS-sensing ability was assessed and validated in cell-free assays and in cell culture.
- Subjects
FLUORESCENT probes; SUPEROXIDES; NEOPLASTIC cell transformation; FLUOROPHORES; CELL culture
- Publication
Chemistry - A European Journal, 2017, Vol 23, Issue 20, p4765
- ISSN
0947-6539
- Publication type
Article
- DOI
10.1002/chem.201700563