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- Title
High‐Sensitive and Background‐Free Coherent Anti‐Stokes Raman Scattering Microscopy Using Delay Modulation.
- Authors
Wu, Fan; Li, Shangyu; Chen, Xun; Yue, Shuhua; Hong, Weili; Wang, Pu
- Abstract
Coherent anti‐Stokes Raman scattering (CARS) microscopy has demonstrated a powerful platform for label‐free, non‐invasive, and chemically specific imaging of biological samples. However, the non‐resonant background limits its ability to detect weak Raman signals. Here, an approach to eliminate the non‐resonant background in CARS using delay modulation (DM) is presented, which is enabled by an acousto‐optic modulator and spectral focusing. The results demonstrate that DM‐CARS reduces the non‐resonant background by 10 times and improves detection sensitivity by 100‐fold over normal CARS. It also shows that DM‐CARS has potential applications in tracking heavy water metabolism in bacteria for antimicrobial susceptibility testing and imaging liver tumor tissues. Furthermore, a handheld DM‐CARS device has also demonstrated for in vivo imaging, which can be beneficial in various applications.
- Subjects
ANTI-Stokes scattering; RAMAN microscopy; MICROBIAL sensitivity tests; BACTERIAL metabolism; DEUTERIUM oxide
- Publication
Laser & Photonics Reviews, 2024, Vol 18, Issue 4, p1
- ISSN
1863-8880
- Publication type
Article
- DOI
10.1002/lpor.202300827