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- Title
Hyaluronidase decreases neutrophils infiltration to the inflammatory site.
- Authors
Fronza, Marcio; Muhr, Cornélia; Silveira, Denise; Sorgi, Carlos; Rodrigues, Stephen; Farsky, Sandra; Paula-Silva, Francisco; Merfort, Irmgard; Faccioli, Lúcia
- Abstract
Objective: To evaluate the in vivo anti-inflammatory potential of bovine hyaluronidase (HYAL) using two different models of acute inflammation. Methods: Air pouches were produced in the dorsal subcutaneous of mice and injected with phosphate saline solution or HYAL. The antiinflammatory action of HYAL was evaluated in carrageenan (Cg)-inflamed air pouches. After 4 and 24 h the cellular influx, protein exudation, cytokines and lipid mediators were evaluated. The action of HYAL on the rolling and adhesion of leukocytes was investigated in the LPS-stimulated mesenteric microcirculation by intravital microscopic. Results: Treatment with HYAL reduced the cellular influx and protein exudation in non-inflamed and inflamed air pouches. HYAL treatment of Cg-inflamed air pouch reduced the production of tumor necrosis factor-alpha (TNF-α), interleukin-8 (IL-8), leukotriene B4 (LTB) and LTC, whereas prostaglandins E (PGE) and D (PGD) concentrations were unchanged. Histological analyses showed that HYAL administration diminished cell infiltration in the air-pouch lining. In LPS-stimulated mesenteric microcirculation, HYAL usage decreased rolling and adhesion of leukocytes, but did not affect the blood vessels diameters. Conclusion: The results demonstrate that HYAL inhibited cellular recruitment, edema formation and pro-inflammatory mediators production, resulting in decreased adherence of leukocytes to blood vessels and tissue infiltration. Our data suggest that HYAL may be considered an effective candidate to ameliorate acute inflammation.
- Subjects
HYALURONIDASES; CATTLE diseases; PROSTAGLANDINS; MICROCIRCULATION; LEUCOCYTES
- Publication
Inflammation Research, 2016, Vol 65, Issue 7, p533
- ISSN
1023-3830
- Publication type
Article
- DOI
10.1007/s00011-016-0935-0