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- Title
Cargo binding and regulatory sites in the tail of fungal conventional kinesin.
- Authors
Seiler, Stephan; Kirchner, Jochen; Horn, Christian; Kallipolitou, Athina; Woehlke, Günther; Schliwa, Manfred
- Abstract
Here, using a quantitative in vivo assay, we map three regions in the carboxy terminus of conventional kinesin that are involved in cargo association, folding and regulation, respectively. Using C-terminal and internal deletions, point mutations, localization studies, and an engineered 'minimal' kinesin, we identify five heptads of a coiled-coil domain in the kinesin tail that are necessary and sufficient for cargo association. Mutational analysis and in vitro ATPase assays highlight a conserved motif in the globular tail that is involved in regulation of the motor domain; a region preceding this motif participates in folding. Although these sites are spatially and functionally distinct, they probably cooperate during activation of the motor for cargo transport.
- Subjects
KINESIN; FUNGI; GENETIC mutation
- Publication
Nature Cell Biology, 2000, Vol 2, Issue 6, p333
- ISSN
1465-7392
- Publication type
Article
- DOI
10.1038/35014022