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- Title
Identification and Functional Analysis of Transcriptome Profiles, Long Non-Coding RNAs, Single-Nucleotide Polymorphisms, and Alternative Splicing from the Oocyte to the Preimplantation Stage of Sheep by Single-Cell RNA Sequencing.
- Authors
Zhang, Zijing; Shi, Qiaoting; Zhu, Xiaoting; Jin, Lei; Lang, Limin; Lyu, Shijie; Xin, Xiaoling; Huang, Yongzhen; Yu, Xiang; Li, Zhiming; Chen, Sujuan; Xu, Zhaoxue; Zhang, Wei; Wang, Eryao
- Abstract
Numerous dynamic and complicated processes characterize development from the oocyte to the embryo. However, given the importance of functional transcriptome profiles, long non-coding RNAs, single-nucleotide polymorphisms, and alternative splicing during embryonic development, the effect that these features have on the blastomeres of 2-, 4-, 8-, 16-cell, and morula stages of development has not been studied. Here, we carried out experiments to identify and functionally analyze the transcriptome profiles, long non-coding RNAs, single-nucleotide polymorphisms (SNPs), and alternative splicing (AS) of cells from sheep from the oocyte to the blastocyst developmental stages. We found between the oocyte and zygote groups significantly down-regulated genes and the second-largest change in gene expression occurred between the 8- and 16-cell stages. We used various methods to construct a profile to characterize cellular and molecular features and systematically analyze the related GO and KEGG profile of cells of all stages from the oocyte to the blastocyst. This large-scale, single-cell atlas provides key cellular information and will likely assist clinical studies in improving preimplantation genetic diagnosis.
- Subjects
ALTERNATIVE RNA splicing; LINCRNA; SINGLE nucleotide polymorphisms; RNA sequencing; OVUM; RNA splicing
- Publication
Genes, 2023, Vol 14, Issue 6, p1145
- ISSN
2073-4425
- Publication type
Article
- DOI
10.3390/genes14061145