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- Title
The role of glutamate in the neurotoxicity due to exposed to sulfite in the prenatal and/or postnatal period in rats.
- Authors
Akçay, Güven; Kencebay, Ceren; Danışman, Betül; Denizaltı, Tuğberk; Saraç, Çiğdem Gökçek; Aslan, Mutay; Derin, Narin
- Abstract
Objective: One of the most widely used food additives is sulfite. It is known that consumption of sulfite over the daily safe level can also lead to toxic effects. It has been demonstrated that sulfite cause to impair the learning and spatial memory by inducing damage to the pyramidal neurons in the hippocampal tissue in rats. In this study, the impacts of sulfite neurotoxicity on glutamate pathway were examined in prenatal and postnatal period. Methods: Experimental groups were separated as; control(C), prenatal(PR), postnatal(PS), prenatal and postnatal(PR+PS). C group were administered standard tap water. Other groups were given 100 mg/kg/day sodiummetabisulfite in drinking water in prenatal, postnatal and both prenatal and postnatal periods. Plasma S-sulfonate level was examined. In the hippocampi of rats Glutamate and glutamine levels were analysed by mass spectrometry and NMDA/AMPA receptor proteins, CaMKII, PKA, CREB protein levels were evaluated by Western-Blotting method. Statistical analyzes were performed by One-Way ANOVA test. Results: In the Morris water maze test, it was seen that the PS and PR+PS groups increased escape latencies compared to the control (p<0.05). Plasma S-sulfonate levels were increased in the PS and PR+PS groups levels compared to the control group (p<0.01). According to the control, glutamate and glutamine concentrations in PR and PR+PS groups (p<0.05), AMPA receptor subtypes in PS and PR+PS groups, NMDA receptor subtypes PR, PS and PR+PS was observed to decrease in groups (p<0.01). Likewise, CaMKII and PKA protein levels decreased in PS and PR+PS groups. Lastly, it was observed that CREB protein level decreased in PS group compared to the C group (p<0.001). Conclusion: It has been shown that sulfite exposure during prenatal and/or postnatal period may have negative effects on learning, glutamate and glutamate receptors.
- Subjects
PUERPERIUM; NEUROTOXICOLOGY; CREB protein; GLUTAMIC acid; AMPA receptors; PYRAMIDAL neurons
- Publication
Anatomy: International Journal of Experimental & Clinical Anatomy, 2019, Vol 13, Issue S1, pS35
- ISSN
1307-8798
- Publication type
Article