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- Title
Identification and Characterization of Process-Related Impurities of Trans-Resveratrol.
- Authors
SIVAKUMAR, Balasubramanian; MURUGAN, Raman; BASKARAN, Annamalai; KHADANGALE, Bhausaheb Pandharinath; MURUGAN, Saravanan; SENTHILKUMAR, Udayampalayam Palanisamy
- Abstract
This article deals with the identification and characterization of process-related impurities of trans-resveratrol (3,5,4'-trihydroxystilbene), which exhibits several health benefits, including cancer prevention. During the synthesis of the bulk drug resveratrol, three new impurities were observed. The impurities were detected using the high-performance liquid chromatographic (HPLC) method, whose area percentages ranged from 0.05 to 0.3%. A systematic study was carried out to characterize them. These impurities were isolated by preparative HPLC and characterized by spectral data, subjected to co-injection in HPLC, and were found to be matching with the impurities present in the sample. LC-MS was performed to identify the mass of these impurities. Based on their spectral data (IR, NMR, and Mass), these impurities were characterized as 2-benzyl-5-[(E)-2-(4-hydroxyphenyl)ethenyl]benzene-1,3-diol [Impurity-B], 3-(benzyloxy)-5-[(E)-2-(4-hydroxyphenyl)ethenyl]phenol [Impurity-C], 5-{(E)-2-[4-(benzyloxy)phenyl]ethenyl}benzene-1,3-diol [Impurity-D). These compounds are not reported earlier as process-related impurities.
- Subjects
RESVERATROL; CANCER prevention; CHEMICAL synthesis; HIGH performance liquid chromatography; LIQUID chromatography-mass spectrometry
- Publication
Scientia Pharmaceutica, 2013, Vol 81, Issue 3, p683
- ISSN
0036-8709
- Publication type
Article
- DOI
10.3797/scipharm.1301-17