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- Title
Aerogels prepared from polymeric β-cyclodextrin and graphene aerogels as a novel host-guest system for immobilization of antibodies: a voltammetric immunosensor for the tumor marker CA 15-3.
- Authors
Jia, Haiyan; Tian, Qingyun; Xu, Jingkun; Lu, Limin; Ma, Xue; Yu, Yongfang
- Abstract
A three-dimensional porous network graphene aerogel (GAs) with large specific area and excellent conductivity was loaded with β-cyclodextrin polymer (Pβ-CD) to serve as a support for immobilization of antibodies. A highly sensitive immunosensor for the cancer marker carbohydrate antigen 15-3 (CA15-3) was designed based on the use of Pβ-CD/GAs. The large specific area of GAs warrants high loading with antibodies, and their excellent electrical conductivity warrants strong electrical signals. Based on the synergistic effect of GAs and Pβ-CD, an immunoassay was designed that is making use of hexacyanoferrate as an electrochemical probe and having a pleasantly low working potential of 0.2 V (vs. SCE). Response is linear in the 0.1 mU mL−1 to 100 U mL−1 activity range, and the lower detection limit is 0.03 mU mL−1 (at S/N = 3). The immunoassay is stable, selective and reproducible. It was applied to the analysis of spiked samples, and results were satisfactory.Schematic of an electrochemical immunoassay for the carbohydrate antigen 15-3. It is based on the use of β-cyclodextrin polymer and a graphene aerogel.
- Subjects
AEROGELS; CYCLODEXTRINS; GRAPHENE oxide; TUMOR markers; METAL foams
- Publication
Microchimica Acta, 2018, Vol 185, Issue 11, p1
- ISSN
0026-3672
- Publication type
Article
- DOI
10.1007/s00604-018-3056-3