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- Title
A SERS-based multiple immuno-nanoprobe for ultrasensitive detection of neomycin and quinolone antibiotics via a lateral flow assay.
- Authors
Shi, Qiaoqiao; Huang, Jie; Sun, Yaning; Deng, Ruiguang; Teng, Man; Li, Qingmei; Yang, Yanyan; Hu, Xiaofei; Zhang, Zhijun; Zhang, Gaiping
- Abstract
The authors describe an ultrasensitive method for simultaneous detection of neomycin (NEO) and quinolones antibiotics (QNS). It is based on the use of (a) two immuno-nanoprobes (a probe for NEO and a probe for QNS), (b) surface-enhanced Raman scattering (SERS) detection, and (c), a portable lateral flow assay (LFA). The two probes consist of gold nanoparticles (AuNPs) conjugated to the Raman active molecule 4-aminothiophenol (PATP), and to monoclonal antibody against NEO (NEO mAb) or against NOR (NOR mAb). Quantitative detection of NEO and QNS was realized via SERS of the PATP-coated AuNPs captured in the test line of a LFA. Under optimized condition, the visual limits of LFA are 10 ng·mL for NEO and 200 ng·mL for NOR, and with LODs down to 0.37 pg·mL and 0.55 pg·mL by using SERS. The NEO test line is not interfered by the NEO analogues gentamycin, streptomycin and tobramycin, but the NOR test line suffers from different degrees of cross-reactivity (CR) to 12 common other QNS, the CRs ranging from 1.5% to 136%. The recoveries of NEO and NOR from spiked milk samples ranged between 86% and 121%, with relative standard deviations (RSD) from 3% to 6%. The method is highly sensitive, accurate and effective. It may be applied to simultaneous detection of NEO and 8 QNS, including NOR, enoxacin, ciprofloxacin, ofloxacin, fleroxacin, marbofloxacin, enrofloxacin, and pefloxacin.
- Subjects
NANO-probe sensors; QUINOLONE antibacterial agents; ANTIBIOTICS; MONOCLONAL antibodies
- Publication
Microchimica Acta, 2018, Vol 185, Issue 2, p1
- ISSN
0026-3672
- Publication type
Article
- DOI
10.1007/s00604-017-2556-x