We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Protective effect of magnolol on TBHP-lnduced injury in h460 cells partially via a p53 dependent mechanism.
- Authors
Li, Hai-bo; Gao, Jian-mei; Ying, Xi-xiang; Wang, Shu; Li, Jian-chun
- Abstract
The aim is to investigate the effect of Magnolol preserved H460 cells from an oxidative agent tert-butylhydroperoxide (TBHP)-induced cell death. Magnolol augmented cell survival ratio after TBHP challenged. The protective action of this drug was more efficacious than that of N-acetylcysteine (NAC) which is a putative antioxidant. DNA damage, detected by the comet assay, was diminished after treatment of Magnolol. The cells viability decreased after treatment with 0.15 mM TBHP for 24 h, accompanied by inducing apoptotic death of the cells. Cytotoxicity and apoptosis induced by TBHP were significantly inhibited or attenuated after pretreatment with 20 μMagnolol. Magnolol contributes to the cells survival through downregulated the p53 phosphorylation and PTEN expression, and upregulated Akt phosphorylation. Taken together, Magnolol was effective against DNA single strand breaks (SSB) formation, cytotoxicity and lipid peroxidation induced by TBHP, and its effects on p53 phosphorylation, PTEN and Akt phosphorylation were due to its antioxidative function, and partially via a p53 dependent mechanism in this protective effects.
- Publication
Archives of Pharmacal Research, 2007, Vol 30, Issue 7, p850
- ISSN
0253-6269
- Publication type
Article
- DOI
10.1007/BF02978836