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- Title
ASSESSMENT OF GENETIC VARIATIONS OF DIVERGENT CITRUS TRISTEZA VIRUS RNA POPULATIONS IN ITALY UPON VECTOR TRANSMISSION PROCESS.
- Authors
Yahiaoui, D.; Djelouah, K.; D'Onghia, A. M.; Catara, A.
- Abstract
Citrus tristeza virus (CTV) causes one of the most disastrous citrus diseases worldwide. In Italy, tristeza outbreaks were reported from Sicily (Catania and Siracusa provinces) and Apulia (Taranto province), where two divergent RNA genomic variants (seedling yellows and mild Isolates), have been found. However, information on the mechanisms involved in the rapid spread of CTV populations and on their genomic changes following vector transmission is still limited. In the present work, representative CTV haplotypes were successfully transmitted by local aphid biotypes. Afterwards, the response of aphid-derived CTV variants to MCA13 strain-specific antibody has been studied and gene mutations have also been assessed by single strand conformation polymorphism (SSCP) of viral p18 and p25 and sequence analysis of the coat protein. Results revealed a high spread potential of CTV variants using Aphis gossypii (about 50%), while transmission efficiency by A. spiraecola (< 3.5%) and Toxoptera aurantii (< 2%) was lower. The Apulian isolate reacted negatively to MCA13 even after transmission, while the Sicilian isolate and its sub-isolates cross-reacted with MCA13. SSCP screening, disclosed the apperance of new haplotypes in the p18 region amplified from the Sicilian A. gossypii-generated subisolates. CP phylogenetic analysis showed high intra-group sequence identities and single nucleotide variations in the isolates consequent to aphid transmission. This finding contributed to understanding the impact of the entomofauna on the homogenity of spreading pattern of two major CTV variants found in Italy.
- Subjects
SICILY (Italy); ITALY; CITRUS tristeza virus; CITRUS tristeza disease; CITRUS diseases &; pests; VIRUS diseases of plants; RNA
- Publication
Journal of Plant Pathology, 2012, Vol 94, Issue 4, pS4.84
- ISSN
1125-4653
- Publication type
Article