We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Haplotype characterization and markers at the barley Mlo powdery mildew resistance locus as tools for marker-assisted selection.
- Authors
Tacconi, G.; Baldassarre, V.; Collins, N. C.; Bulgarelli, D.; Stanca, A. M.; Valè, G.
- Abstract
Recessive mlo alleles of the barley Mlo gene confer resistance to almost all known isolates of the powdery mildew fungal pathogen targeting barley (Hordeum vulgare). To characterize haplotypes present in the Mlo chromosomal region of cultivated Mlo and mlo barley genotypes, weconducted a polymorphism search in 3 predicted low-copy sequence regions adjacent to the Mlo gene by examining a sample of 4 Mlo and 3 mlo cultivars. Eight single-nucleotide polymorphisms (SNPs) and 1 insertiondeletion (indel) were detected, and easy to use PCR-based markers were developed for typing the SNPs. The PCR markers were used to characterize a collection of 46 Mlo and 25 mlo barley cultivars, identifying 3 distinct mlo-11 haplotypes, 1 mlo-9 haplotype, and 4 Mlo haplotypes. We summarized the haplotype and marker information obtained here and in a previous study to help breeders identify strategies for mlo marker-assisted selection. The ability of the markers to identify mlo-resistant genotypes in segregating populations was demonstrated using 2 resistance-characterized F2 populations derived by 3-way crosses.
- Subjects
BARLEY; PHYTOPLASMAS; GENETIC markers; PATHOGENIC fungi; HEIRLOOM varieties (Plants); PLANT mutation breeding; GENETIC polymorphisms
- Publication
Genome, 2006, Vol 49, Issue 8, p864
- ISSN
0831-2796
- Publication type
Article
- DOI
10.1139/G06-044