We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Expression and translation of the COX-1b gene in human cells - no evidence of generation of COX-1b protein.
- Authors
Reinauer, Christina; Censarek, Petra; Kaber, Gernot; Weber, Artur-Aron; Steger, Gerhard; Klamp, Thorsten; Schrör, Karsten
- Abstract
Cyclooxygenase 1b ( COX-1b) is a splice variant of COX-1, containing a retained intron 1 within the signal peptide sequence. COX-1b mRNA is found in many species, but the existence of a functionally active protein, which is possibly related to different species-dependent lengths of intron 1, is controversially discussed. The human intron 1 comprises 94 bp, and the resulting frameshift at the intron 1-exon 2 junction creates a premature stop codon. Nevertheless, full-length human COX-1b protein expression, including translated intron 1 and the signal peptide, has been reported and was explained by a frameshift repair. In this study, the fate of COX-1b mRNA in a human overexpression system is analyzed. Independent of the hypothetical frameshift repair mechanism, the splicing of the COX-1b intron 1, resulting in COX-1 mRNA and removal of the signal peptide during protein maturation, with subsequent generation of a COX-1 protein is demonstrated.
- Subjects
HUMAN cell membranes; GENE expression; GENETIC translation; CYCLOOXYGENASES; SIGNAL peptides; MESSENGER RNA; SPECIES
- Publication
Biological Chemistry, 2013, Vol 394, Issue 6, p753
- ISSN
1431-6730
- Publication type
Article
- DOI
10.1515/hsz-2012-0309