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- Title
Determination of Sulfated Peptides by Differential Iodination.
- Authors
Schepky, Andreas G.; Schmidt, André M.; Schmidt, Thomas; Schulz-Knappe, Peter; Forssmann, Wolf-Georg
- Abstract
A sequential approach was developed to label tyrosine sulfate and peptides containing tyrosine sul-fate selectively. Amino acids and peptides containing tyrosine and tyrosine sulfate were first iodinated using chloramine-T-method. Reaction products were determined by RP-HPLC. Mono- and biiodination of tyrosine and several model peptides was achieved within 120 s incubation time, lodination of free tyrosine sulfate and sulfated cholecystokinin26-33 was less than 5%. After desulfation of the reaction products with 1 N HCI successful radioiodination of desulfated tyrosine was carried out whereas tyrosine did incorporate radioactive iodine only 10%. As shown by RP-HPLC specific labeling of tyrosine sulfate containing peptides with 125iodine was achieved.
- Publication
Biological Chemistry, 1994, Vol 375, Issue 3, p201
- ISSN
1431-6730
- Publication type
Article