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- Title
Heat-shock response is associated with decreased production of interleukin-6 in murine aortic vascular smooth muscle cells.
- Authors
InKyeom Kim; Heung-Mook Shin; Woonyi Baek
- Abstract
Heat shock has been known to change cellular responses to noxious stimuli by inducing heat-shock proteins (Hsps). We hypothesized that a heat-shock response modulates cytokine production in murine aortic vascular smooth muscle cells (VSMCs). VSMCs were exposed to 44°C for 15-60 min, and subjected to interleukin-1ß (IL-1ß) or tumor necrosis factor a (TNFa), which induced interleukin-6 (IL-6) production. Expression of Hsps was examined with immunoblots, immunocytochemistry, or enzyme-linked immunosorbent assay (ELISA), and that of IL-6 with reverse transcription-polymerase chain reaction (RT-PCR) or ELISA. Heat shock (44°C for 45 min) induced Hsp72 in VSMCs at 4 h and elicited its maximal expression at 8 h after the end of heat shock. Treatment with IL-1ß increased IL-6 transcription in VSMCs up to 24 h in an incubation time-dependent manner. Treatment with IL-1ß or TNFa caused a concentration-dependent increase in IL-6 production in culture medium, which was attenuated by heat shock. Although treatment with Hsp72 or Hsp60 alone did not significantly affect basal IL-6 release into culture medium statistically, cotreatment with IL-1ß and Hsp72, but not Hsp60 or boiled Hsp72, decreased IL-1ß-induced IL-6 production in culture medium. Introduction of Hsp72, but not Hsp60, into VSMCs decreased IL-1ß-induced IL-6 production in culture medium. These results indicate that the heat-shock response transcriptionally attenuated production of IL-6 in murine aortic VSMCs.
- Subjects
HEAT shock proteins; INTERLEUKIN-6; LABORATORY mice; CYTOKINES; TUMOR necrosis factors; ENZYME-linked immunosorbent assay; POLYMERASE chain reaction
- Publication
Naunyn-Schmiedeberg's Archives of Pharmacology, 2005, Vol 371, Issue 1, p27
- ISSN
0028-1298
- Publication type
Article
- DOI
10.1007/s00210-004-1007-5