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- Title
Inhibition of hepatitis B virus replication by small interference RNA induces expression of MICA in HepG2.2.15 cells.
- Authors
Kai-Fu Tang; Min Chen; Jing Xie; Guan-Bin Song; Yi-Song Shi; Qi Liu; Zhe-Chuan Mei; Steinle, Alexander; Hong Ren
- Abstract
Hepatitis B virus (HBV) replicates in most tumor tissues of patients with HBV-associated hepatocellular carcinoma (HCC). In the present study, we have shown that the expression of HBV in the HCC cell lines, HepG2 and Huh7, down-regulated the expression of MHC class I-related molecule A (MICA), a ligand of the NKG2D receptor. Inhibition of HBV expression by small interference RNAs (siRNAs) in HepG2.2.15, a cell line that constitutively expresses HBV, induced up-regulation of MICA. The up-regulation of MICA increased the lysis of HepG2.2.15 cells by NK cells. Our results suggest that HBV compromises the innate immune system in HCC patients and that inhibition of HBV replication by siRNAs may enhance the antitumor immune response.
- Subjects
HEPATITIS B virus; LIVER cancer; CELL lines; SMALL interfering RNA; PATIENTS; KILLER cells
- Publication
Medical Microbiology & Immunology, 2009, Vol 198, Issue 1, p27
- ISSN
0300-8584
- Publication type
Article
- DOI
10.1007/s00430-008-0101-6