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- Title
Cells on a Chip - the Use of Electric Properties for Highly Sensitive Monitoring of Blood-Derived Factors Involved in Angiotensin II Type 1 Receptor Signalling.
- Authors
Rothermel, Andrée; Kurz, Randy; Rüffer, Markus; Weigel, Winnie; Jahnke, Heinz-Georg; Sedello, Anna K.; Stepan, Holger; Faber, Renaldo; Schulze-Forster, Kai; Robitzki, Andrea A.
- Abstract
Background: We developed a highly sensitive cardiomyocyte based screening system for the non-destructive electronic detection of chronotropic drugs and tissue-secreted factors involved in AT1 receptor-mediated cardiovascular diseases. Methods: For this purpose we cultured spontaneously beating neonatal rat cardiomyocytes on microelectrode arrays (MEAs), and tested the optimised, stable culture parameters for a reproducible real-time recording of alterations in contraction frequency. After the evaluation of culture parameters, computer-based electronic measurement systems were used for counting of contractions by recording of the field potential of cardiomyocytes. Results: Using the biosensor, angiotensin II, the predominant ligand of the AT1 receptor, was detected at very low concentrations of 10-11 M via altered contractions of cardiomyocytes. Moreover, we demonstrated that cardiomyocyte coupled microarrays allow the detection of blood-derived low concentrated anti-AT1 receptor autoimmune antibodies of pregnant women suffering from preeclampsia. Conclusion: This study demonstrates the first well-suited electrophysiological recording of cardiomyocytes on multielectrode arrays as a benefit for functional biomonitoring for the detection of AT1 receptor/ligand interactions and other marker proteins in sera directed to cardiovascular diseases. Copyright © 2005 S. Karger AG, Basel
- Subjects
HEART cells; CARDIOVASCULAR diseases; MICROELECTRODES; AUTOIMMUNE diseases; IMMUNOGLOBULINS
- Publication
Cellular Physiology & Biochemistry (Karger AG), 2005, Vol 16, Issue 1-3, p051
- ISSN
1015-8987
- Publication type
Article
- DOI
10.1159/000087731