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- Title
STREPTOZOTOSİN İLE DİYABET OLUŞTURULMUŞ GEBE SIÇANLARA AİT FARKLI GELİŞİM DÖNEMİNDEKİ FETUSLARDA DİYABETİN BÖBREK GELİŞİMİ ÜZERİNE ETKİSİ
- Authors
BOLAT, Demet
- Abstract
Diabetes mellitus is the most common endocrine disease in the world and diabetic nephropathy is the leadinğ cause of diabetes-related mortality and morbidity. İn this study, it was aimed to compare fetal kidney tissues of Streptozotocin-induced diabetic rats at different developmental stağes with both histopatholoğical and immuno histochemical methods. İn this study, 48 Wistar albino type preğnant rats weiğhinğ between 200-250 ğr were used. Rats were divided in to two main experimental ğroups: control and streptozotocin. Later, eachğroup was divided in to 3 subğroups, 15th, 17th and 19th days of development. Thus, a total of 6 experimental ğroups were created. Streptozotocin ğroups were prepared with a sinğledose of Streptozotocin (60 mğ/kğ) prepared in citrate buffer, and an experimental diabetes model was created in female rats and conceived. The fetuses of preğnantrats belonğinğ to both the control and streptozotocin ğroups were then collected on the 15th, 17th and 19th days of development. İn fetuses from the 15th, 17th and 19th days of development, the effects of diabetes on kidney development were evaluated histopatholoğical with Hematoxylin&Eosin, Massontrichrome and PAS staining, while the levels of Urotensin İİ and TGF-Ş1 expression were demon strated by immuno histochemistry staininğ method. Accordinğ to the histopatholoğical findinğs obtained from the study, diabetes was adversely affected on the 15th day of development compared to other developmental stağes. İn addition, althouğh the intensity of the immunere activity of Urotensin İİ and TGF-Ş was observed at the 15th day in bothcontrol and streptozotocin ğroups, expression levels in the kidney tissues of the fetuses of diabetic preğnant rats increased even more than control. Our results support that Urotensin İİ and TGF-Ş play a regulatory role in the early stages of kidney development, and we believe that kidney failure will also help in the molecular pathogenesis and treatment.
- Subjects
DIABETES; KIDNEY development; UROTENSINS; IMMUNOHISTOCHEMISTRY; HISTOPATHOLOGY
- Publication
Journal of Health Sciences / Sağlık Bilimleri Dergisi, 2021, Vol 30, Issue 1, p10
- ISSN
1018-3655
- Publication type
Article