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- Title
Synthetic biology, genome mining, and combinatorial biosynthesis of NRPS-derived antibiotics: a perspective.
- Authors
Baltz, Richard H.
- Abstract
Combinatorial biosynthesis of novel secondary metabolites derived from nonribosomal peptide synthetases (NRPSs) has been in slow development for about a quarter of a century. Progress has been hampered by the complexity of the giant multimodular multienzymes. More recently, advances have been made on understanding the chemical and structural biology of these complex megaenzymes, and on learning the design rules for engineering functional hybrid enzymes. In this perspective, I address what has been learned about successful engineering of complex lipopeptides related to daptomycin, and discuss how synthetic biology and microbial genome mining can converge to broaden the scope and enhance the speed and robustness of combinatorial biosynthesis of NRPS-derived natural products for drug discovery.
- Subjects
NONRIBOSOMAL peptide synthetases; SYNTHETIC biology; ANTIBIOTICS; BIOSYNTHESIS; MICROBIAL genomes; METABOLITE synthesis; HYBRID enzymes
- Publication
Journal of Industrial Microbiology & Biotechnology, 2018, Vol 45, Issue 7, p635
- ISSN
1367-5435
- Publication type
Article
- DOI
10.1007/s10295-017-1999-8