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- Title
Identification of a newly isolated erythritol-producing yeast and cloning of its erythritol reductase genes.
- Authors
Deng, Huihui; Han, Ye; Liu, Yuanyuan; Jia, Wei; Zhou, Zhijiang
- Abstract
A new erythritol-producing yeast (strain BH010) was isolated in this study. Analysis of the D1/D2 domain of the 26S rDNA sequence, the ITS/5.8S rDNA sequence, and the 18S rDNA sequence allowed the taxonomic position of strain BH010 to be discussed and it was identified and named Moniliella sp. BH010. Physiological characteristics were described. Scanning electron micrography clearly indicated that the cells were cylindrical to elliptical with an average size of 5 × 10 μm when growing in liquid medium, and that pseudohyphae and blastoconidia were observed when cultivated in agar plates. The erythritol reductase genes were cloned, sequenced, and analyzed. BLAST analysis and multiple sequence alignment demonstrated that erythritol reductase genes of Moniliella sp. BH010 shared very high homology with that of Trichosporonoides megachiliensis SNG-42 except for the presence of introns. The deduced amino acid sequences showed high homology to the aldo-keto reductase superfamily.
- Subjects
YEAST genetic engineering; RECOMBINANT DNA; ALCOHOL; REDUCTASE genetics; AMINO acids; MOLECULAR cloning
- Publication
Journal of Industrial Microbiology & Biotechnology, 2012, Vol 39, Issue 11, p1663
- ISSN
1367-5435
- Publication type
Article
- DOI
10.1007/s10295-012-1162-5