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- Title
婴幼儿配方乳粉中低聚半乳糖含量检测方法的前处理优化.
- Authors
王巧云; 夏 璇; 张 敏; 李光辉; 马 镇
- Abstract
Objective To optimize the pretreatment conditions of the international method for the determination of GOS to improve the accuracy of detection results. Methods Ten min ultrasonic treatment was added to the current pretreatment method and centrifugation was carried out for sedimentation proteinto. The optimal centrifugation conditions were determined: 10000 r/min, 10 min. In addition, the eluent ratio of gradient elution was optimized. Five different characteristic peaks of GOS were found by ion chromatography detection and quantitative external standard method. GOS2 was verified as GOS quantitative peak to calculate GOS content. Results The correlation coefficient (r2) of the standard curve was greater than 0.995 in the range of 5-100 μg/mL. The recovery rates were 100.0% to 120.0% and the relative standard deviations were 0.22% to 6.40% (n=2) at the standard levels of 0.6, 1.2, 2.0, 3.0 and 4.0 g/kg. Conclusion The optimized method is more effective in sample extraction, better in separation of sample peak and better in recovery and repeatability, and higher in accuracy, which can satisfy the requirement of determination of GOS in infant formula milk powder.
- Publication
Journal of Food Safety & Quality, 2020, Vol 11, Issue 8, p2540
- ISSN
2095-0381
- Publication type
Article