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- Title
Droplet-Based Microfluidic Platform for High Spatiotemporal Resolved Single-Cell Signaling Profiling.
- Authors
Sun, Yingnan; Tian, Qingqing; Liu, Yongshu; Xing, Kunming; Li, Yuyan; Liu, Yumin; Zhang, Shusheng
- Abstract
A small indentation embedded in a microchannel creates a surface energy well (SEW) for a confined droplet due to surface energy release. Inspired by this, we developed a SEW-based microfluidic platform to realize high spatiotemporal-resolved signal profiling at the single-cell level applying droplet stimulus on a single chip. The method allows for controlled droplet replacement within only 3 s with almost 100% exchange efficiency, reliable single-cell patterning of adherent cells and successive treatment of adherent cells with reagent droplets. Furthermore, the PDGFR/Akt pathway served as a model system for evaluating the performance of the SEW-based method in determining the effects of ligand stimulation duration (3 s to 3 min) on receptor phosphorylation. The novel strategy offers a general platform for probing the temporal dynamics of single cells, as well for monitoring rapid chemical reactions in various applications.
- Subjects
SURFACE energy; CHEMICAL reactions; SIGNALS &; signaling; CELL aggregation; SEWING
- Publication
Chemosensors, 2022, Vol 10, Issue 12, p521
- ISSN
2227-9040
- Publication type
Article
- DOI
10.3390/chemosensors10120521