We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Cow Milk Extracellular Vesicle Effects on an In Vitro Model of Intestinal Inflammation.
- Authors
Mecocci, Samanta; Ottaviani, Alessio; Razzuoli, Elisabetta; Fiorani, Paola; Pietrucci, Daniele; De Ciucis, Chiara Grazia; Dei Giudici, Silvia; Franzoni, Giulia; Chillemi, Giovanni; Cappelli, Katia
- Abstract
Extracellular vesicles (EVs) are lipid bilayer nano-dimensional spherical structures and act mainly as signaling mediators between cells, in particular modulating immunity and inflammation. Milk-derived EVs (mEVs) can have immunomodulatory and anti-inflammatory effects, and milk is one of the most promising food sources of EVs. In this context, this study aimed to evaluate bovine mEVs anti-inflammatory and immunomodulating effects on an in vitro co-culture (Caco-2 and THP-1) model of intestinal inflammation through gene expression evaluation with RT-qPCR and cytokine release through ELISA. After establishing a pro-inflammatory environment due to IFN-γ and LPS stimuli, CXCL8, IL1B, TNFA, IL12A, IL23A, TGFB1, NOS2, and MMP9 were significantly up-regulated in inflamed Caco-2 compared to the basal co-culture. Moreover, IL-17, IL-1β, IL-6, TNF-α release was increased in supernatants of THP-1. The mEV administration partially restored initial conditions with an effective anti-inflammatory activity. Indeed, a decrease in gene expression and protein production of most of the tested cytokines was detected, together with a significant gene expression decrease in MMP9 and the up-regulation of MUC2 and TJP1. These results showed a fundamental capability of mEVs to modulate inflammation and their potential beneficial effect on the intestinal mucosa.
- Subjects
EXTRACELLULAR vesicles; GOAT milk; INTESTINES; COWS; INTESTINAL mucosa; ANTI-inflammatory agents
- Publication
Biomedicines, 2022, Vol 10, Issue 3, p570
- ISSN
2227-9059
- Publication type
Article
- DOI
10.3390/biomedicines10030570