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- Title
Tissue-specific mechanical and geometrical control of cell viability and actin cytoskeleton alignment.
- Authors
Dong Wang; Wenfu Zheng; Yunyan Xie; Peiyuan Gong; Fang Zhao; Bo Yuan; Wanshun Ma; Yan Cui; Wenwen Liu; Yi Sun; Matthieu Piel; Wei Zhang; Xingyu Jiang
- Abstract
Different tissues have specific mechanical properties and cells of different geometries, such as elongated muscle cells and polygonal endothelial cells, which are precisely regulated during embryo development. However, the mechanisms that underlie these processes are not clear. Here, we built an in vitro model to mimic the cellular microenvironment of muscle by combining both mechanical stretch and geometrical control. We found that mechanical stretch was a key factor that determined the optimal geometry of myoblast C2C12 cells under stretch, whereas vascular endothelial cells and fibroblasts had no such dependency. We presented the first experimental evidence that can explain why myoblasts are destined to take the elongated geometry so as to survive and maintain parallel actin filaments along the stretching direction. The study is not only meaningful for the research on myogenesis but also has potential application in regenerative medicine.
- Subjects
TISSUE-specific antigens; CYTOSKELETAL proteins; MYOBLAST transfer therapy; CYTOSKELETON formation; STRESS fibers (Cytology); BIOMEDICAL engineering
- Publication
Scientific Reports, 2014, p1
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/srep06160