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- Title
The Cytocam video microscope. A new method for visualising the microcirculation using Incident Dark Field technology.
- Authors
Hutchings, Sam; Watts, Sarah; Kirkman, Emrys
- Abstract
We report a newmicrocirculatory assessment device, the Braedius Cytocam, an Incident Dark Field (IDF) video microscope, and compare it with a precursor device utilising side stream dark field (SDF) imaging. METHODS: Time matched measurements were made with both devices from the sublingual microcirculation of pigs subjected to traumatic injury and hemorrhagic shock at baseline and during a shock phase. Images were analysed for vessel density, microcirculatory flow and image quality. RESULTS: There were no differences in density or flow data recorded from the two devices at baseline [TVD IDF 14.2±2.4/TVD SDF 13.2±2.0, p 0.17] [MFI IDF 3 (2.8-3.0)/MFI SDF 3 (2.9-3.0), p 0.36] or during the shock state [TVD IDF 11.64±3.3/TVD SDF 11.4±4.0 p = 0.98] [MFI IDF 1.9 (0.6-2.7)/MFI SDF 1.7 (0.3-2.6) p 0.55]. Bland and Altman analysis showed no evidence of significant bias. Vessel contrast was significantly better with the IDF device for both capillaries [17.1±3.9 (IDF) v 3.4±3.6 (SDF), p = 0.0006] and venules [36.1±11.4 (IDF) v 26.4±7.1 (SDF) p 0.014] CONCLUSION: The Braedius Cytocam showed comparable vessel detection to a precursor device during both baseline and low flow (shock) states.
- Subjects
MICROSCOPY; MICROCIRCULATION; HEMORRHAGIC shock; IMAGE quality analysis; LABORATORY swine
- Publication
Clinical Hemorheology & Microcirculation, 2016, Vol 62, Issue 3, p261
- ISSN
1386-0291
- Publication type
Article
- DOI
10.3233/CH-152013