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- Title
Estimation of genetic relationship of wheat germplasm against cereal cyst nematodes using RAPD technique.
- Authors
Erum, Y. I.; Shahina, F.
- Abstract
Investigations were conducted at molecular level, to estimate genetic diversity among wheat germplasm (20 cultivars/lines) in relation to their response against cereal cyst nematodes Heterodera avenae, through Random Amplified Polymorphic DNA (RAPD) technique. A total of 589 bands were generated using 14 primers with an average of 28.8 bands/genotype. Maximum percentage of polymorphic loci was 92.86% for genotype TJ-83. Inferences have been made regarding bioassay and molecular characterization that the most diverse and resistant genotype against CCN was Moomal-2002 as compared to the rest of the genotypes studied and the most effective loci to screen diversity was OPA-09.
- Subjects
CROP germplasm; HETERODERA avenae; RAPD technique; WHEAT genetics; GENOTYPES; LOCUS in plant genetics
- Publication
Sarhad Journal of Agriculture, 2016, Vol 32, Issue 4, p193
- ISSN
1016-4383
- Publication type
Article
- DOI
10.18681/pjn.v34.i02.p193