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- Title
Determination of unprocessed genetically modified soybean in foods using simplex and duplex real-time PCR with an internal standard.
- Authors
Harikai, Naoki; Saito, Shin; Tanaka, Atsuko; Kinoshita, Kenji
- Abstract
We developed a method using an internal standard to determine the amount of unprocessed genetically modified organisms (GMO) in foods as GMO weight per weight of total food (w/wtf) using real-time PCR. Food samples were spiked with an internal standard, a ColE1 plasmid, and DNA was extracted from the samples using a silica membrane-type kit and analysed using real-time PCR. The relationship between the GMO amount and the copy number ratio of the transgene to ColE1 in 0.1–5% w/wtf GM soybean powders was found to have a correlation coefficient ( r) of 0.97. GMO was quantified in food samples spiked with GM soybean (final amount 0.5% w/wtf GMO). The average GMO amount ranged from 0.35% to 0.63% w/wtf. The results show that our method should be useful for quantifying unprocessed GMO in foods. We also developed a duplex assay, which is simpler and more accurate than the simplex assay.
- Subjects
SOYBEAN; UNPROCESSED foods; GENETICALLY modified foods; DNA; TRANSGENES; POLYMERASE chain reaction
- Publication
International Journal of Food Science & Technology, 2009, Vol 44, Issue 9, p1778
- ISSN
0950-5423
- Publication type
Article
- DOI
10.1111/j.1365-2621.2009.01996.x