We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Up-regulated expression of Toll-like receptors mRNAs in peripheral blood mononuclear cells from patients with systemic lupus erythematosus.
- Authors
Komatsuda, A.; Wakui, H.; Iwamoto, K.; Ozawa, M.; Togashi, M.; Masai, R.; Maki, N.; Hatakeyama, T.; Sawada, K.
- Abstract
Recent studies in animal models for systemic lupus erythematosus (SLE) have shown that Toll-like receptors (TLR-7 and TLR-9) and interferon (IFN)-α are involved in the pathogenesis of murine lupus. Recent studies using flow cytometry have also shown increased expression of TLR-9 in peripheral blood mononuclear cells (PBMCs) from SLE patients. In this study, we performed quantitative real-time reverse transcription–polymerase chain reaction analyses of PBMCs from 21 SLE patients and 21 healthy subjects, to estimate TLR2, TLR3, TLR4, TLR5, TLR7, TLR8, TLR9, IFN-α and LY6E (a type I IFN-inducible gene) mRNA expression levels. Expression levels of TLR2, TLR7, TLR9, IFN-α and LY6E mRNAs in SLE patients were significantly higher than those in healthy controls. Expression levels of TLR7 and TLR9 mRNAs correlated with that of IFN-α mRNA in SLE patients. These results suggest that up-regulated expression of TLR7 and TLR9 mRNAs together with increased expression of IFN-α mRNA in PBMCs may also contribute to the pathogenesis of human lupus.
- Subjects
MESSENGER RNA; SKIN diseases; ANTIVIRAL agents; AUTOIMMUNE diseases; ANTINEOPLASTIC agents; DNA polymerases
- Publication
Clinical & Experimental Immunology, 2008, Vol 152, Issue 3, p482
- ISSN
0009-9104
- Publication type
Article
- DOI
10.1111/j.1365-2249.2008.03646.x