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- Title
Regulation of the <em>chIA</em> locus of <em>Escherichia coli</em> K12: involvement of molybdenum cofactor.
- Authors
Baker, K. P.; Boxer, D. H.
- Abstract
The <em>chlA</em> locus encodes functions required for the biosynthesis of the molybdopterin part of the molybdenum cofactor Mutants, carrying gene fusions at the <em>chlA</em> locus, which place β-galactosidase expression under the control of the <em>chlA</em> promoter, have been isolated employing <em>λplacMu1</em> as the mutagen, The mutants exhibited β-galactosidase expression which was greatly enhanced when grown anaerobically. Secondary mutations at the <em>chlB, D, E or G loci</em> did not affect the high level of expression. The <em>fnr</em> gene product was not required for the anaerobic expression. Bacteriophage λ transducing phages were isolated which carried the φ(chlA-lac) mutations and were used to construct <em>chlA.../φ(chlA-lac)</em> merodiploids. The merodiploids exhibited a much lower level of expression but showed the same characteristics as strains carrying lac fusions to the single chromosomal <em>chlA</em> locus. Genetic evidence is presented which strongly suggests that the molybdenum cofactor is a repressor of <em>chlA</em> expression. The anaerobic enhancement of <em>chlA</em> expression is mediated via a mechanism that is distinct from the molybdenum cofactor effect.
- Subjects
MOLYBDENUM; BIOSYNTHESIS; GENE fusion; GENETIC mutation; GENES; ESCHERICHIA coli
- Publication
Molecular Microbiology, 1991, Vol 5, Issue 4, p901
- ISSN
0950-382X
- Publication type
Article
- DOI
10.1111/j.1365-2958.1991.tb00764.x