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- Title
A chemo-enzymatic oxidation cascade to activate C–H bonds with in situ generated H<sub>2</sub>O<sub>2</sub>.
- Authors
Freakley, Simon J.; Kochius, Svenja; van Marwijk, Jacqueline; Fenner, Caryn; Lewis, Richard J.; Baldenius, Kai; Marais, Sarel S.; Opperman, Diederik J.; Harrison, Susan T. L.; Alcalde, Miguel; Smit, Martha S.; Hutchings, Graham J.
- Abstract
Continuous low-level supply or in situ generation of hydrogen peroxide (H2O2) is essential for the stability of unspecific peroxygenases, which are deemed ideal biocatalysts for the selective activation of C–H bonds. To envisage potential large scale applications of combined catalytic systems the reactions need to be simple, efficient and produce minimal by-products. We show that gold-palladium nanoparticles supported on TiO2 or carbon have sufficient activity at ambient temperature and pressure to generate H2O2 from H2 and O2 and supply the oxidant to the engineered unspecific heme-thiolate peroxygenase PaDa-I. This tandem catalyst combination facilitates efficient oxidation of a range of C-H bonds to hydroxylated products in one reaction vessel with only water as a by-product under conditions that could be easily scaled. Continuous low-level supply or in situ generation of hydrogen peroxide (H2O2) is essential for the stability of unspecific peroxygenases. Here, the authors demonstrate that AuPd / TiO2 can generate sufficient H2O2 for the engineered unspecific heme-thiolate peroxygenase PaDa-I to oxidise a range of C-H bonds.
- Subjects
OXIDATION; CHEMICAL bonds; HYDROGEN peroxide; ENZYMES; HYDROXYLATION
- Publication
Nature Communications, 2019, Vol 10, Issue 1, pN.PAG
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-019-12120-w