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- Title
miRNA-205 Nanoformulation Sensitizes Prostate Cancer Cells to Chemotherapy.
- Authors
Nagesh, Prashanth K. B.; Chowdhury, Pallabita; Hatami, Elham; Boya, Vijaya K. N.; Kashyap, Vivek K.; Khan, Sheema; Hafeez, Bilal B.; Chauhan, Subhash C.; Jaggi, Meena; Yallapu, Murali M.
- Abstract
The therapeutic application of microRNA(s) in the field of cancer has generated significant attention in research. Previous studies have shown that miR-205 negatively regulates prostate cancer cell proliferation, metastasis, and drug resistance. However, the delivery of miR-205 is an unmet clinical need. Thus, the development of a viable nanoparticle platform to deliver miR-205 is highly sought. A novel magnetic nanoparticle (MNP)-based nanoplatform composed of an iron oxide core with poly(ethyleneimine)-poly(ethylene glycol) layer(s) was developed. An optimized nanoplatform composition was confirmed by examining the binding profiles of MNPs with miR-205 using agarose gel and fluorescence methods. The novel formulation was applied to prostate cancer cells for evaluating cellular uptake, miR-205 delivery, and anticancer, antimetastasis, and chemosensitization potentials against docetaxel treatment. The improved uptake and efficacy of formulations were studied with confocal imaging, flow cytometry, proliferation, clonogenicity, Western blot, q-RT-PCR, and chemosensitization assays. Our findings demonstrated that the miR-205 nanoplatform induces significant apoptosis and enhancing chemotherapeutic effects in prostate cancer cells. Overall, these study results provide a strong proof-of-concept for a novel nonviral-based nanoparticle protocol for effective microRNA delivery to prostate cancer cells.
- Subjects
DOCETAXEL; AGAR; CANCER chemotherapy; CELL lines; DRUG delivery systems; DRUG resistance in cancer cells; ELECTROPHORESIS; FLOW cytometry; IMMUNIZATION; IMMUNOASSAY; IRON compounds; METASTASIS; MICROSCOPY; NANOPARTICLES; POLYETHYLENE; POLYMERASE chain reaction; PROSTATE tumors; WESTERN immunoblotting; DRUG development; ETHYLENE glycols; REVERSE transcriptase polymerase chain reaction; GENE expression profiling; MICRORNA
- Publication
Cancers, 2018, Vol 10, Issue 9, p289
- ISSN
2072-6694
- Publication type
Article
- DOI
10.3390/cancers10090289