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- Title
One Divinyl Reductase Reduces the 8-Vinyl Groups in Various Intermediates of Chlorophyll Biosynthesis in a Given Higher Plant Species, But the Isozyme Differs between Species.
- Authors
Pingrong Wang; Chunmei Wan; Zhengjun Xu; Pingyu Wang; Wenming Wang; Changhui Sun; Xiaozhi Ma; Yunhua Xiao; Jianqing Zhu; Xiaoling Gao; Xiaojian Deng
- Abstract
Divinyl reductase (DVR) converts 8-vinyl groups on various chlorophyll intermediates to ethyl groups, which is indispensable for chlorophyll biosynthesis. To date, five DVR activities have been detected, but adequate evidence of enzymatic assays using purified or recombinant DVR proteins has not been demonstrated, and it is unclear whether one or multiple enzymes catalyze these activities. In this study, we systematically carried out enzymatic assays using four recombinant DVR proteins and five divinyl substrates and then investigated the in vivo accumulation of various chlorophyll intermediates in rice (Oiyza sativa), maize (Zea mays), and cucumber (Cucumis sativus). The results demonstrated that both rice and maize DVR proteins can convert all of the five divinyl substrates to corresponding monovinyl compounds, while both cucumber and Arabidopsis (Arabidopsis thalinna) DVR proteins can convert three of them. Meanwhile, the OsDVR (Os03g22780)-inactivated 824ys mutant of rice exclusively accumulated divinyl chlorophylls in its various organs during different developmental stages. Collectively, we conclude that a single DVR with broad substrate specificity is responsible for reducing the 8-vinyl groups of various chlorophyll intermediates in higher plants, but DVR proteins from different species have diverse and differing substrate preferences, although they are homologous
- Subjects
CHLOROPHYLL; PLANT enzymes; PLANT proteins; PLANT protein genetics; ARABIDOPSIS thaliana genetics
- Publication
Plant Physiology, 2013, Vol 161, Issue 1, p521
- ISSN
0032-0889
- Publication type
Article
- DOI
10.1104/pp.112.208421