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- Title
GM-CSF secretion in primary cultures of normal and cancerous human renal cells.
- Authors
Stephens, Nicole D.; Barton, Sandra L.; Smith, Anthony Y.; Paul, Ralph W.; Neidhart, James A.; Griffith, Jeffrey K.
- Abstract
Rates of secretion of granulocyte-macrophage colony stimulating factor (GM-CSF) were measured in 50 primary cell cultures derived from cancerous and normal human kidneys. Mean rates of GM-CSF secretion measured by TF-1 cell proliferation assay (N = 21) and by ELISA (N = 31) were 2.5 and 7.K ng/106 cells/24 hr, respectively, There was no significant difference between the mean rates of GM-CSF .secretion by cancerous and normal renal cells, GM-CSF was also secreted by primary renal cell cultures grown in serum-free medium and by renal cell lines, GM-CSF mRNA was detected by RT-PCR in cultured renal cells, but not in undissociated kidney tissue. Rates of GM-CSF secretion were reduced up to 99% under conditions where the cellular density or substratum more closely resembled the in vivo environment. Some cultured human renal carcinoma cells (RCC) secreted GM-CSF al levels that occasionally overlapped the levels produced by the GM-CSF gene-modified human RCC vaccine now in phase I trial. The data indicate that GM-CSF is not expressed in vivo, and .hat stable GM-CSF secretion is induced by the dissociation and culture of human renal cells.
- Subjects
GRANULOCYTE-macrophage colony-stimulating factor; CELL culture; CANCER cells; KIDNEYS; NEPHROLOGY; CLINICAL biochemistry
- Publication
Kidney International, 1996, Vol 50, Issue 3, p1044
- ISSN
0085-2538
- Publication type
Article
- DOI
10.1038/ki.1996.407