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- Title
Something old, something new, a DNA preparation procedure for long-read genomic sequencing.
- Authors
Wang, Hongjun; Xu, Sen
- Abstract
Extracting high-molecular-weight DNA is critical for successful long-read DNA sequencing. Here, we present a DNA preparation procedure combining the CTAB DNA extraction protocol and a short-read eliminator kit, which consistently produces high yields of high-molecular-weight DNA in the freshwater microcrustacean Daphnia. This method only requires common chemicals and equipment, providing researchers a viable alternative to DNA extraction kits that often require specialized tools.
- Subjects
DNA; DNA sequencing; DAPHNIA; CHEMICAL apparatus; DNA polymerases
- Publication
Genome, 2022, Vol 65, Issue 1, p53
- ISSN
0831-2796
- Publication type
Article
- DOI
10.1139/gen-2021-0029