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- Title
Blockade of glial-derived neurotrophic factor in laryngeal muscles promotes appropriate reinnervation.
- Authors
Hernandez‐Morato, Ignacio; Tewari, Ishan; Sharma, Sansar; Pitman, Michael J.
- Abstract
<bold>Objectives/hypothesis: </bold>Synkinetic reinnervation of the laryngeal muscles is one of the causes of the poor functional recovery after a recurrent laryngeal nerve (RLN) injury. Glial-derived neurotrophic factor (GDNF) is elevated in rat laryngeal muscles during RLN reinnervation. The specific aim of this investigation was to evaluate the effect of anti-GDNF on RLN reinnervation.<bold>Methods: </bold>Anti-GDNF antibody was injected into the posterior cricoarytenoid (PCA) 3 days following RLN transection and anastomosis. Larynges were harvested at 7, 14, 28, 56, and 112 days post injury (DPI). Prior to sacrifice, the vocal fold mobility was assessed. Immunostaining to identify neuromuscular junctions was used to evaluate the extent of axonal reinnervation of the PCA, lateral thyroarytenoid (LTA), and medial thyroarytenoid (MTA).<bold>Results: </bold>After anti-GDNF injection into PCA, RLN reinnervation in all muscles was altered when compared to the controls. PCA innervation was delayed. At 7 DPI, only a few axons made synapses in the PCA. In contrast, axons prematurely innervated the LTA and MTA when compared to controls. Innervation was similar to controls at 56 and 112 DPI. Vocal fold motion was enhanced in 10 of 24 animals studied.<bold>Conclusions: </bold>After injection of anti-GDNF into the PCA, early arriving axons bypass the PCA and enter the LTA. Later arriving axons innervate the PCA and MTA. Vocal fold function is improved as compared to controls. Anti-GDNF injection into the PCA influences the pattern of reinnervation and may result in less synkinetic, more functional innervation.<bold>Level Of Evidence: </bold>NA Laryngoscope, 126:E337-E342, 2016.
- Subjects
LARYNGEAL nerve palsy; NEUROTROPHINS; IMMUNOSTAINING; AXONS; RNA analysis; WOUNDS &; injuries; NEURAL physiology; LARYNGEAL nerve injuries; ANIMALS; BIOLOGICAL models; IMMUNOGLOBULINS; INTRAMUSCULAR injections; LARYNGEAL muscles; LARYNGEAL nerves; NERVE tissue; NERVOUS system regeneration; RATS; VOCAL cords; INNERVATION
- Publication
Laryngoscope, 2016, Vol 126, Issue 10, pE337
- ISSN
0023-852X
- Publication type
journal article
- DOI
10.1002/lary.25953