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- Title
晒田环境胁迫对克氏原螯虾卵巢发育相关生化指标和激素的影响.
- Authors
袁畅; 李蔚; 黄玉英; 何苹萍; 郑玉斯; 李鑫; 卢智发; 张圣杰; 李文红; 彭金霞; 王大鹏
- Abstract
[ Objective] The purpose of the study was to investigate the changes of biochemical indexes and hormones related to the ovarian development of Procambarus clarkii under the sun-field environmental stress and the effects on ovarian development, to provide theoretical basis for the improvement and standardization of the production operation of sun-field environmental stress to promote the maturation of the ovary of P. clarkii, and to promote the sustainable development of the P. clarkii industry. [ Method] P. clarkii were palced in 2 ponds with an area of 1300 m2 as the sun-field group and the control group. Using draining in the sun-field group as the starting point of the experiment, the sunfield group was drained for 10 days and the control group was cultured normally. Female P. clarkii in the 2 groups were collected at O, 3, 7 and 10 days of sun-field environmental stress, respectively. The number of samples was 30 at each time, and the ovaries, hepatopancreas and serum were measured and weighed for the detection of biochemical indices related to ovarian development [ alkaline phosphatase ( AKP / ALP), acid phosphatase ( ACP), glutathione peroxidase ( GSH-Px), prostaglandin F2u ( PGF2a), prostaglandin E2 ( PGE2)], sex steroid hormones [Estrone, 17!3-estradiol (17!3-E2), testosterone (T), progesterone (PROG)], the hyperglycemic family hormones [chromogranulin ( CHH), gonadal inhibitory hormone ( GIH), molting inhibitory hormone ( MIH), mandibular organ inhibitory hormone ( MOIH) ], and the hormones related to ovarian development [ Ecdysone, methylfamesylate ( MF), Cortisol] and were comparatively analyzed. [Result] With the increase of time, the ovarian maturation rate of female P. clarkii in the sun-field group was higher than that of the control group, and the ovarian developmental stage was mainly distributed in stage IV. The results of the biochemical index test showed that at O days of sun-field stress, the contents of AKP/ ALP, ACP, GSH-Px, PGF2u and PGE2 in different tissues of the sun-field group were significantly lower than those of the control group ( P < 0. 05, the same as below) or were not significantly different from those of the control group ( P > 0. 05, the same as below). At 3 and 7 days of sun-field stress, the contents of the biochemical indexes in different tissues of female P. clarkii in the sun-field group were significantly higher than those of the control group or were not significantly different from the control group. At 10 days of sun-field stress, the contents of the biochemical indexes in different tissues of female P. clarkii in the sun-field group were significantly higher than those of the control group. The results of the sex steroid hormone test showed that at O and 3 days of sunfield stress, the contents ofEstrone, 17!3-E2, T and PROG in female P. clarkii in the sun-field group were significantly higher than those in the control group or there was no significant difference between the two groups. At 7 and 10 days of sun-field stress, neutral steroid hormones in female P. clarkii in the sun-field group were significantly higher than those in the control group. The results of hyperglycemic family hormone test showed that at 0 days of sun-field stress, the contents of CHH, GIH, MIH and MOIH were significantly higher than those of the control group. At 3 and 7 days of sun-field stress, the contents of hyperglycemic family hormones in the sun-field group were significantly higher than those of the control group, lower than those of the control group, or had no significant difference with the control group. At 10 days of sun-field stress, the levels of hyperglycemic family hormones in the sun-field group were significantly lower than those in the control group or were not significantly different from those in the control group. The results of ovarian development-related hormones test showed that the contents of Ecdyone, MF and Cortisol in the sun-field group were significantly higher than, lower than or not significantly different from those in the control group at 0 days of sun-field stress. At 3 days of sun-field stress, the ovarian development-related hormones in the sunfield group were significantly higher than or not significantly different from those in the control group. at 7 and 10 days of sun-field stress, ovarian development-related hormones were significantly higher in the sun-field group than in the control group. [ Conclusion] The sun-field environmental stress can promote the maturation of the ovaries of P. clarkii, accelerate the changes of biochemical indexes and hormones related to ovarian development The effect of promoting ovarian maturation reaches its peak at 7 -10 days of sun-field stress.
- Subjects
PROCAMBARUS clarkii; HORMONES
- Publication
Southwest China Journal of Agricultural Sciences, 2023, Vol 36, Issue 10, p2292
- ISSN
1001-4829
- Publication type
Article
- DOI
10.16213/j.cnki.scjas.2023.10.026