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- Title
Ligand interaction scan: a general method for engineering ligand-sensitive protein alleles.
- Authors
Erster, Oran; Eisenstein, Miriam; Liscovitch, Mordechai
- Abstract
The ligand interaction scan (LIScan) method is a general procedure for engineering small molecule ligand–regulated forms of a protein that is complementary to other 'reverse' genetic and chemical-genetic methods for drug-target validation. It involves insertional mutagenesis by a chemical-genetic 'switch', comprising a genetically encoded peptide module that binds with high affinity to a small-molecule ligand. We demonstrated the method with TEM-1 β-lactamase, using a tetracysteine hexapeptide insert and a biarsenical fluorescein ligand (FlAsH).
- Subjects
BIOCHEMISTRY; LIGANDS (Biochemistry); PROTEIN engineering; CHEMICAL modification of proteins; COORDINATION compounds
- Publication
Nature Methods, 2007, Vol 4, Issue 5, p393
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth1046