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- Title
EXAFS reveals a structural zinc binding site in the bovine NADH-Q oxidoreductase
- Authors
Giachini, Lisa; Francia, Francesco; Boscherini, Federico; Pacelli, Consiglia; Cocco, Tiziana; Papa, Sergio; Venturoli, Giovanni
- Abstract
Abstract: The metal content of bovine NADH-Q oxidoreductase determined by inductively-coupled plasma atomic-emission spectroscopy reveals the presence of about one atom of zinc per molecule of flavin mononucleotide. We applied Zn K-edge extended X-ray absorption fine structure spectroscopy (EXAFS) to investigate the local structure of the bound zinc ion and to identify the nature of the coordinating residues. The EXAFS spectrum is consistent with a structured zinc binding site. By combining information from first-shell analysis and from metalloprotein data bases putative binding clusters have been built and fitted to the experimental spectrum using ab initio simulations. The best fitting binding cluster is formed by two histidine and two cysteine residues arranged in a tetrahedral geometry.
- Subjects
EXTENDED X-ray absorption fine structure; SPECTRUM analysis; OPTICS; X-ray spectroscopy
- Publication
FEBS Letters, 2007, Vol 581, Issue 29, p5645
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2007.11.019