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- Title
Peroxiredoxin-4 interacts with and regulates the thromboxane A<sub>2</sub> receptor
- Authors
Giguère, Patrick; Turcotte, Marie-Eve; Hamelin, Emilie; Parent, Audrey; Brisson, Jessy; Laroche, Geneviève; Labrecque, Pascale; Dupuis, Gilles; Parent, Jean-Luc
- Abstract
Abstract: We identified peroxiredoxin-4 (Prx-4) as a protein interacting with the β isoform of the thromboxane A2 receptor (TPβ) by yeast two-hybrid analysis. Prx-4 co-immunoprecipitated constitutively with TPβ in HEK293 cells. The second and third intracellular loops as well as the C-terminus of TPβ interacted directly with Prx-4. Co-expression of Prx-4 caused a 60% decrease in cell surface expression of TPβ. Prx-4 and TPβ predominantly co-localized in the endoplasmic reticulum. Co-expression of Prx-4 in cells treated with H2O2 targeted TPβ for degradation. We show for the first time an interaction between a receptor involved in oxidative stress and Prx-4, an anti-oxidative enzyme.
- Subjects
HEMAGGLUTININ; OXIDATIVE stress; ENDOPLASMIC reticulum; ENZYME-linked immunosorbent assay
- Publication
FEBS Letters, 2007, Vol 581, Issue 20, p3863
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2007.07.011