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- Title
Synergetic inhibition of metal ions and genistein on α-glucosidase
- Authors
Wang, Yufang; Ma, Lin; Li, Zhong; Du, Zhiyun; Liu, Zhong; Qin, Jiangke; Wang, Xiaodong; Huang, Zhishu; Gu, Lianquan; Chen, Albert S.C.
- Abstract
Inhibition of metal ions and synergetic inhibition of metal ions/genistein on α-glucosidase activity has been investigated. We have examined the inhibitory effect of Cu2+, Ni2+, Mg2+, Fe2+, Hg2+, Zn2+, Ca2+, Pb2+, Ag+, V5+, V4+ and Mn2+ ions. The results show that the nature of the inhibition was reversible, slow-binding, non-competitive, and the <f>Ki</f> values of some ions such as Cu2+, Ni2+ and Zn2+ range from 10-5 to 10-6 M. Moreover, synergetic inhibitory effect of metal ions and genistein on α-glucosidase were studied with kinetics method. It is concluded that the inhibitory effect was much greater when both of them were added to the reactant solution simultaneously than that they were added, respectively, which suggests that the inhibitors seem to bind to the different sites of α-glucosidase at the same time. Furthermore, the mechanism of the synergetic inhibition was examined by spectrophotometry.
- Subjects
METAL ions; CHROMIUM ions; GLUCOSIDASES; GLYCOSIDASES
- Publication
FEBS Letters, 2004, Vol 576, Issue 1/2, p46
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2004.08.059