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- Title
Antibacterial, Anti-Chlamydial, and Cytotoxic Activities of a Marine Snail ( Hexaplex trunculus) Phospholipase A2: an In Vitro Study.
- Authors
Zarai, Zied; Gharsallah, Houda; Hammami, Adnane; Mejdoub, Hafedh; Bezzine, Sofiane; Gargouri, Youssef-Talel
- Abstract
In order to report pharmacological characterization of marine snail ( Hexaplex trunculus) hepatopancreatic phospholipase A (mSDPLA), we have talked for the first time the antimicrobial activity against different pathogenic bacterial strains, anti-chlamydial activity as well as its cytotoxic activity against McCoy cell lines. mSDPLA, showed a high level of activity towards Gram-positive bacteria as Staphylococcus aureus and Staphylococcus epidermidis. Whereas Gram-negative bacteria, unfortunately, exhibited a higher resistance, mSDPLA was also found to have a strong cytotoxic activity, causing significant morphological alterations of the McCoy cell lines surfaces and to be a hinder to the proliferation. Moreover, mSDPLA proved to have a very potent anti-chlamydial activity. Over 95 % inhibition of chlamydial inclusions were obtained at a concentration of 10 μg/ml of mSDPLA after 24 h postinfection. Interestingly, at a concentration of 10 μg/ml of mSDPLA, the proliferation of McCoy cells was not affected. Approximately 50 % inhibition of cell growth was obtained with a concentration of 37 μg/mL of mSDPLA. mSDPLA could be considered as an excellent candidate for the development of a new anti-infective agent. This enzyme showed significant antimicrobial activities.
- Subjects
ANTIBACTERIAL agents; PHOSPHOLIPASE A2; DEACYLASES; CYTOTOXINS; CELL lines
- Publication
Applied Biochemistry & Biotechnology, 2012, Vol 168, Issue 4, p877
- ISSN
0273-2289
- Publication type
Article
- DOI
10.1007/s12010-012-9826-1