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- Title
The −930A>G polymorphism of the CYBA gene is associated with premature coronary artery disease. A case-control study and gene-risk factors interactions.
- Authors
Niemiec, Pawel; Nowak, Tomasz; Iwanicki, Tomasz; Krauze, Jolanta; Gorczynska-Kosiorz, Sylwia; Grzeszczak, Wladyslaw; Ochalska-Tyka, Anna; Zak, Iwona
- Abstract
Reactive oxygen species (ROS) are involved in the pathogenesis of atherosclerosis and coronary artery disease (CAD). NADPH oxidases are the main source of ROS in the vasculature. p22phox is a critical component of vascular NADPH oxidases and is encoded by the CYBA (cytochrome b alpha) gene. The −930A>G CYBA polymorphism (rs9932581:A>G) modulates the activity of the CYBA promoter, and influences CYBA transcriptional activity. The aim of the present study was to analyze a possible association between the −930A>G polymorphism and CAD and to search for gene-traditional risk factors interactions. 480 subjects were studied: 240 patients with premature CAD, 240 age and sex matched blood donors. The −930A>G polymorphism was genotyped using the TaqMan Pre-designed SNP Genotyping Assay (Applied Biosystems). The −930G allele carrier state was a risk factor for CAD (OR 2.03, 95 % CI 1.21-3.44, P = 0.007). A synergistic effect of the −930G allele with overweight/obesity (BMI ≥ 25) and cigarette smoking was found. The estimated CAD risk for BMI ≥ 25 and the −930G allele interaction was about 160 % greater than that predicted by assuming additivity of the effects, and about 40 % greater for interaction of cigarette smoking and the −930G allele. Overweight/obesity was a risk factor for CAD only in the −930G allele carriers ( P < 10) but not in the AA homozygotes ( P = 1.00). In conclusion the −930A>G CYBA polymorphism is associated with CAD in the Polish population. The −930G allele carriers are particularly at risk of consequences of obesity and tobacco smoke exposure.
- Subjects
GENETIC polymorphisms; ATHEROSCLEROSIS; CORONARY disease; NADPH oxidase; REACTIVE oxygen species; HEART disease risk factors; OBESITY; TOBACCO smoke
- Publication
Molecular Biology Reports, 2014, Vol 41, Issue 5, p3287
- ISSN
0301-4851
- Publication type
Article
- DOI
10.1007/s11033-014-3191-9