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- Title
Simultaneous separation of intracellular and extracellular lactate NMR signals of human erythrocytes.
- Authors
Kohler, Götz; Rost, Florian; Seelig, Joachim
- Abstract
Intracellular/extracellular lactate (Lac) distribution has been determined before in human and animal erythrocytes (red blood cells [RBCs]) with various methods. However, all previous methods determine intra- and extracellular Lac separately or indirectly. Now, 13C-NMR spectroscopy has been used to monitor intra- and extracellular Lac simultaneously in intact RBCs. Isolated human RBCs were incubated with [3-13C]-Lac, [3-13C]-pyruvate (Pyr), and [1-13C]-glucose (Gluc). A distortionless enhancement by polarization transfer (DEPT) sequence was used (TR = 3.3 s, N = 128) to monitor the 13C-NMR resonances in both compartments. The intra- and extracellular methyl group resonances of Lac and Pyr were clearly separated by 9.6 Hz and 7.0 Hz, respectively, under normoxic conditions due to the RBC chemical-shift effect. The results show that the chemical-shift effect of RBCs is convenient to monitor intra- and extracellular Lac simultaneously in intact RBCs under normoxic conditions. Magn Reson Med 58:213-217, 2007. © 2007 Wiley-Liss, Inc.
- Publication
Magnetic Resonance in Medicine, 2007, Vol 58, Issue 2, p213
- ISSN
0740-3194
- Publication type
Article
- DOI
10.1002/mrm.21309