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- Title
Neuron-specific expression of atp6v0c2 in zebrafish CNS.
- Authors
Chung, Ah-Young; Kim, Myoung-Jin; Kim, Dohyun; Bang, Sangsu; Hwang, Sun Wook; Lim, Chae Seung; Lee, Sanggyu; Park, Hae-Chul; Huh, Tae-Lin
- Abstract
Vacuolar ATPase (V-ATPase) is a multi-subunit enzyme that plays an important role in the acidification of a variety of intracellular compartments. ATP6V0C is subunit c of the V0 domain that forms the proteolipid pore of the enzyme. In the present study, we investigated the neuron-specific expression of atp6v0c2, a novel isoform of the V-ATPase c-subunit, during the development of the zebrafish CNS. Zebrafish atp6v0c2 was isolated from a genome-wide analysis of the zebrafish mib ta52b mutant designed to identify genes differentially regulated by Notch signaling. Whole-mount in situ hybridization revealed that atp6v0c2 is expressed in a subset of CNS neurons beginning several hours after the emergence of post-mitotic neurons. The ATP6V0C2 protein is co-localized with the presynaptic vesicle marker, SV2, suggesting that it is involved in neurotransmitter storage and/or secretion in neurons. In addition, the loss-of-function experiment suggests that ATP6V0C2 is involved in the control of neuronal excitability. Developmental Dynamics 239:2501-2508, 2010. © 2010 Wiley-Liss, Inc.
- Publication
Developmental Dynamics, 2010, Vol 239, Issue 9, p2501
- ISSN
1058-8388
- Publication type
Article
- DOI
10.1002/dvdy.22383